The present study investigated metabolic changes and their relationship to vascular histopathological changes in the atherosclerotic arteries of rabbits with alloxan induced diabetes. Essential oils samples were obtained from the aerial parts of the plant by hydrodistillation and analyzed by gcms. Comparative toxicity of alloxan, nalkylalloxans and ninhydrin to isolated pancreatic islets in vitro. Hypoglycemic and antioxidant effect of oleuropein in alloxandiabetic rabbits article in life sciences 7812. Alloxan and streptozotocin are toxic glucose analogues that preferentially accumulate in pancreatic beta cells via the glut2 glucose transporter. This study was to create a longterm rabbit model of diabetes mellitus for medical studies of up to one year or longer and to evaluate the effects of chronic hyperglycemia on damage of major organs. Alloxan induced rats,anti diabetes, diabetes, ipomoea batatas, phytochemical analysis. The optimum effective dose of alloxan was found to be 140 mgkg bw for induction of stable diabetes in long evan rats. They were randomly and equally divided into the control group c, n30, alloxaninduced group with dm dm, n30 and allopurinoltreated group with dm allo, n30. Diabetes is a disease that constitutes multiple sources of free radicals, thus oxidative stress is expected to have a double impact. The first group 5 rabbits was used as control and fed with standard chow and tap water. When they finish that diet, ordinary food was given to those.
The effect, however, was more pronounced in diabetic animals in which administration for 4 weeks after alloxan induced diabetes, significantly reduced blood glucose levels. May 24, 2014 mice administered a single intraperitoneal injection of alloxan monohydrate at a dose of 150 mgkg showed progression of typei diabetes symptoms figure 2. The methanolic whole plant extract at high dose 500 mgkg exhibited significant antihyperglycemic activity than whole plant extract at low dose 300 mgkg in diabetic rats. Similarly, alazzawie and alhamdani 19 reported that dietary oleuropein in alloxan diabetic rabbits reduced plasma mda levels to the level of nondiabetic animals. On microscopic study these observers found necrosis of. The mechanisms of alloxan and streptozotocininduced. Alloxaninduced and insulinresistant diabetes mellitus. Schuh wholeplant in normal and alloxaninduced diabetic rabbits.
Alloxan, sometimes referred to as alloxan hydrate, refers to the organic compound with the formula ocnhco 2 coh 2. The ethanol extract of fenugreek seeds significantly decreases the plasma malondialdehyde mda from 2. We used alloxan to induce diabetes in rats and rabbits for screening the antidiabetic activity of test compounds. Sar407899 dosedependently relaxed the precontracted corpora cavernosa in all species, with similar potency and efficacy in healthy vs diabetic rats, wky vs shr rats, healthy vs diabetic rabbits. The evaluation of soybean extracts in alloxan induced diabetic rabbits. This study undertaken to investigate the was beneficial effect of vitamin c and zinc on some biochemical parameter when induced diabetic in rabbits by alloxanrabbits groups. In 1937 jacobs 1 found that alloxan 2 the ureide of mesoxalic acid produced fatal hypoglycemia when injected intravenously into rabbits. Alloxan monohydrate is used to induce diabetes mellitus in albino rats in the dose of 120mgkg i. Alloxan has been administered in single or multiple doses, through different routes intraperitoneal. Alloxan induced diabetic rabbits as animal model for diabetes.
Sir, we read with interest the article titled anomalies in alloxaninduced diabetic model. Blood samples 10ml were collected from each animals at end of experiment by the heart cardiac puncture. Alloxan monohydrate is a common drug used for developing experimental diabetes in animals, as streptozotocin, though less toxic than alloxan is considerably costlier. In the alloxan induced diabetic rats showed that extract at 500 mgkg decreased significantly fasting plasma glucose level by 61. The name is derived from allantoin, a product of uric acid excreted by the fetus into the allantois, and oxaluric acid derived from oxalic acid and urea, found in urine. The severe diabetes produced by alloxan results in blood sugar levels equivalent to a total pancreatectomy, hence sulphonylureas such. Similar experiments conducted by dunn, sheehan and mcletchie 3 confirmed the hypoglycemic action of alloxan. Hypoglycemic and antioxidant effect of oleuropein in alloxan. The effect of aepe leaf extract on blood glucose, insulin, hemoglobin and. Alloxan diabetes and streptozotocin diabetes figure 1 shows a schematic diagram of the tetraphasic and triphasic blood glucose responses induced by alloxan and streptozotocin, respectively, when injected 22.
Effect of trigonelline and ethanol extract of iraqi. If the inline pdf is not rendering correctly, you can download the pdf file here. A total of 90 male and female japanese rabbits weighing 1. Research article alloxan induced diabetes mellitus in wistar. The mechanism of alloxan and streptozotocin action in b.
Streptozotocin induced acute clinical effects in rabbits. Experimental model of induction of diabetes mellitus in rats. Attenuation of oxidative damage in alloxan induced diabetic. Study the effect of phytoesterol of ceratoina female rabbits.
Metformin was orally administered at 50 mgkg, while the extract was administered both to normal and diabetic rabbits at 100, 200 and 300 mgkg. Diabetic atherosclerosis was induced in rabbit iliofemoral arteries by injecting alloxan 100 mgkg, injuring the arteries using a balloon, and feeding with a 0. Alloxan induced diabetic rabbits as animal model for. Attenuation of oxidative damage in alloxan induced. Histopathological abnormalities of prolonged alloxaninduced.
Alloxan dose of 120, 140, 160 and 180 mg kg 1 body weight was given intraperitonealy to experimental rats in groups of 6 animals for each dose. In remaining ten rabbits, diabetes mellitus was induced by alloxan monohydrate 150 mgkg by intraperitoneal administration. Introduction diabetes mellitus diabetes is a group of metabolic diseases in which a person has high blood sugar, either because the body does not produce enough insulin, or because cells do not respond to the insulin that is. Diabetes mellitus were induced in twenty four starved pregnant female rabbits by giving alloxan injected by one ml size syringe and in dose 150mg\kg for three days. Alloxan induced and insulinresistant diabetes mellitus affect semen parameters and impair spermatogenesis in male rats 108 african journal of reproductive health african journal of reproductive health vol. The mechanisms of alloxan and streptozotocininduced diabetes. Effect of trigonella foenum methi on fasting blood glucose levels in. Extracts were administered daily for 7d at doses of 200 and 400mgkg, p. Antidiabetic activity of bauhinia forficata extracts in. Alloxan, diabetes mellitus, long evans rat, induced diabetes, toxicity. Alloxan is one of the frequently used betacytotoxic agents for the induction of type1 diabetes mellitus in animal models and is the drug of choice in rabbits.
It is better to standardize it first by jain et al. The present paper is an extension of this study of the mechanism of alloxan hypoglycemia. Altered glucose metabolism and hypoxic response in alloxan. The present study investigated metabolic changes and their relationship to vascular histopathological changes in the atherosclerotic arteries of rabbits with alloxaninduced diabetes. The results showed a significant improvement in the stress induced oxidation parameters by alloxaninduced diabetes in rabbits. Alloxaninduced diabetes is a form of insulindependent diabetes mellitus that occurs as a result of alloxan administration or injection to animals. The present study described the phytochemical profile of lavandula stoechas essential oils, collected in the area of aindraham northwest of tunisia, as well as their protective effects against alloxaninduced diabetes and oxidative stress in rat. Since then, alloxan diabetes has been commonly utilized as an animal model of insulindependent diabetes mellitus iddm. The alloxan model of diabetes was first described in rabbits by dunn, sheehan and mcletchie in 1943. Bailey cc, bailey ot, leech rs 1946 diabetes mellitus in rabbits. These agents selectively destroy the langerhans islet. Pdf pathomorphological effects of alloxan induced acute. K p v o o evaluation and optimization of effectivedose. Diabetes mellitus was induced by a 120 mgkg body weight intravenous injection of alloxan.
On microscopic study these observers found necrosis of all the islets of langerhans while the. Alloxaninduced diabetes, a common model for evaluating. Alloxan induced diabetic rabbits n 28 were divided. The present study was carried out to evaluate the antidiabetic activity of vinca rosea methanolic whole plant extracts in alloxan induced diabetic rats for 14 days. Alloxan exerts its diabetogenic action when it is administered parenterally. The mechanism of alloxan and streptozotocin action in b cells. Alloxan and streptozotocin are toxic glucose analogues that preferentially. Antidiabetic activity of vinca rosea extracts in alloxan. Xanthine oxidase inhibitor allopurinol prevents oxidative. The responses are accompanied by corresponding inverse changes in plasma insulin and sequential ultrastructural. Changes in blood glucose f profile of alloxaninduced diabetic rabbits for 26. Antidiabetic effect of ipomoea batatas in normal and alloxan. Antidiabetic effect of ipomoea batatas in normal and. The anhydrous derivative ocnhco 2 co is also known as well as a dimeric derivative.
The effect of the injection of diabetogenic doses of alloxan on blood sugar levels. Since oxidative stress is mediated by hyperglycemiainduced generation of free radical, it is supposed that compounds with hypoglycemic and antioxidative properties would be useful antidiabetic agents. Histopathological abnormalities of prolonged alloxaninduced diabetes mellitus in rabbits, international. In vivo penile erection length induced by drugs was measured in conscious rabbits. These are some of the earliest known organic compounds.
Alloxaninduced rats,antidiabetes, diabetes, ipomoea batatas, phytochemical analysis. Effects of aqueous extract of alfalfa on hyperglycemia and. This study aimed to create diabetic animal models and to provide an exact and detailed account of alloxan induced diabetes in rabbits. As seen from the data in figure 2, fasting blood glucose levels were reduced versus vehicle treatment following two weeks of oral treatment with glyburide 39. Erectile properties of the rhokinase inhibitor sar407899 in. Blood glucose lowering activity of aloe based composition. Research article alloxan induced diabetes mellitus in. They also exhibit a variety of biological activities. Dec 18, 2007 alloxan has two distinct pathological effects. Mar 23, 2012 diabetes was induced by streptozotocin or alloxan injection.
The present study was conducted to investigate antidiabetic efficacy of extracts of nigella sativa linn. Streptozotocin stz is used for induction of type1 diabetes mellitus in animal models. The responses are accompanied by corresponding inverse changes in. Effect of trigonelline and ethanol extract of iraqi fenugreek. Another 12 agematched normal rabbits were used as controls. In animals, it can be induced by partial pancreatectomy or by the administration of diabetogenic drugs such as alloxan, streptozotocin, ditizona and antiinsulin serum. Alloxaninduced diabetes mellitus how is alloxaninduced. Diabetes mellitus, rabbits, alloxan abstract diabetes mellitus dm a disease which is characterized by hyperglycemia, lipoprotein abnormalities and oxidative stress. Alloxan induced diabetes is a form of insulindependent diabetes mellitus that occurs as a result of alloxan administration or injection to animals. Antidiabetic activity of aqueous extract of leptospermum.
It has been successfully induced in a variety of animal species. Nov 29, 2015 models for insulin dependent diabetes mellitus iddm alloxan induced diabetes principlemechanism interacts with sulfhydryl groups and inhibit it following its uptake by the beta cells, alloxan interacts with sulfhydryl containing cellular components, particularly enzymes known to be essential for beta cell function. Alloxan has been widely used to induce experimental dm in animals such as rabbits, mice and dogs with different grades of disease severity by varying the dose of alloxan used 8 causing alloxan. Modulation of hematological indices of normal and alloxan. Its betacytotoxic action results in sudden release of insulin leading to.
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